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Immunocytochemical labeling of Sema-3A and its receptors in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-Sema-3A (C-terminal) (SP1221), anti-NRP1 (NP221), and anti-Plexin A1 (PP1301). The antibodies were detected using appropriate secondary antibody conjugated to either DyLight® 488 or DyLight® 594.