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Western blot analysis of human A549 (lane 1), MDA-MB-231 (lane 2), and MeWo (lane 3) cell lysates, as well as human breast (lane 4), lung (lane 5), skin (lane 6), and brain (lane 7) tissue lysates. The blot was probed with mouse monoclonal anti-CD44 (CM0241) at 1:2000.