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Western blot analysis of FXYD5 protein expression. Immunoprecipitates using FM0311 FXYD5 antibody only (lane 1) or FM0311 with A431 lysate (lane 2) or A431 input only (lane 3). Human cell lysates MDA-MB-231 (lane 4) or MeWo (lane 5). The blot was probed with mouse monoclonal FXYD5 (FM0291) at 1:1000.