Western blot using native conditions to examine NM0161 immunoprecipitates (IP) and whole lysates. The NM0161 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), The blot was probed with anti-Na+/K+ ATPase β3 NM0161 (lanes 1-3) and anti-Na+/K+ ATPase β3 NM0201 (lanes 4-6). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.