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Western blot analysis of human A431 (lanes 1 & 2), MDA-MB-231 (lanes 3 & 4), and MCF-7 (lanes 5 & 6) cell lysates under native (lanes 1, 3 & 5) and denatured (lanes 2, 4 & 6) conditions. The blots were probed with mouse monoclonal anti-Trop-2 (TM0051) at 1:1000 (lanes 1-6).