ECM Biosciences
ECM Logo Bar
Search :
Technical Data Sheet

AK6340

image
image
image
Akt Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
AM1141 Wu, C. et al. (2012) Cell. 148(5):973. WB: mouse embryonic fibroblasts
AM1141 Paterniti, I. et al. (2011) Eur J Neurosci. 33(8):411. WB: mouse spinal cord
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
AM1011 Wei, H., Vander Heide, R. (2010) AJP Heart Circ. 298:H152. WB: rat myocardium
AM1011 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
AM1011 Moylan, J.S. et al. (2008) AJP Cell. Phys. 295:986. WB: mouse C2C12
Kit Summary
The Akt phospho-regulation antibody sampler kit can be used to detect Akt activation via Ser-473 phosphorylation, as well as Akt inhibition through Thr-34 phosphorylation. The kit also includes a monoclonal antibody to monitor the total Akt expression levels and secondary reagents for detection of these antibodies.
image
image
Enlarge

Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).

image
Enlarge

Western blot analysis of A431 cells, serum starved overnight (20 µg/lanes 1 & 3) and calyculin A (100 nM) treated for 30 minutes (20 µg/lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).

image
Background
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Background References
Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
Jones, P.F. et al. (1991) Proc Natl Acad Sci. USA 88:4171.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Buffer and Storage
AP1001 and AM1011 are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide, and AM1141 is provided in PBS with 0.02% azide. The secondary reagents are supplied in PBS, 50% glycerol, 1 mg/ml BSA, and no azide. Store at –20°C. Stable for 1 year.
imageRelated Products
AK6340
Akt Phospho-Regulation Antibody Sampler Kit

AL9171
A431 Lysate

MK6050
MAP Kinase Activation Antibody Sampler Kit

PM2531
PI3 Kinase, p85 (C-terminal region) Mouse Monoclonal

AX1005
phospho-Akt (Thr-34) Blocking Peptide

MS3011
Anti-Mouse Ig:DyLight® 488 Goat Polyclonal

MS3031
Anti-Mouse Ig:DyLight® 594 Goat Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

RS3241
Anti-Rabbit Ig Light-Chain Specific, unconjugated Mouse Monoclonal

New Releases
FM0131
image
Fibronectin (Cell/Heparin2 Binding region)
Mouse Monoclonal
image
IK6700
image
Integrin β family
Antibody Sampler Kit
image
CM0331
image
CD55 (Extracellular region)
Mouse Monoclonal
image