ECM Logo Bar
Search :
Technical Data Sheet

AL9171

image
image
image
A431
Lysate
Price
Size

$75
100 μl

image
Application
Dilution


WB
20 μl/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
A431 cells express approximately 106 epidermal growth factor (EGF) receptors at the cell surface. Upon stimulation with EGF, A431 cells exhibit a dramatic increase in phosphorylation of EGF receptors followed by activation of major cell signaling pathways, such as PKB/Akt and MAP kinase pathways. Downstream of these cell signaling pathways, a variety of cytoskeletal, cytoplasmic, and nuclear proteins become phosphorylated at different time points after EGF stimulation.

Confluent cultures of A431 cells grown in DMEM containing 10% Fetal bovine serum were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Buffer and Storage
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
imageRelated Products
AL9101
A431 + Calyculin A (30min) Lysate

AL9201
A431 EGF Control Lysate

AL9401
A431 Pervanadate Control Lysate

New Releases
TM0051
image
Trop-2/TACSTD2 (Extracellular region)
Mouse Monoclonal
image
FM0131
image
Fibronectin (Cell/Heparin2 Binding region)
Mouse Monoclonal
image
SK6710
image
Sox2 Thr-118/Lys-119 Regulation
Antibody Sampler Kit
image