ECM Logo Bar
Search :
Technical Data Sheet

AM2981

image
image
image
Annexin A2 (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck
36 kDa

image
image
Enlarge

Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).

image
Enlarge

Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight® 594.

image
Application
Dilution


ELISA
1:2000


ICC
1:50


IHC
1:50


IP
1:50


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Moylan, J. et al. (2014) Redox biology 2:910. WB: C2C12 myoblast
Gilliam, L.A. et al. (2011) AJP Lung Cell Mol Phys 300:L225. IHC: mouse diaphragm
image
Background
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.


Background References
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Immunogen
Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6730
Annexin Family Antibody Sampler Kit

AM0091
Annexin A2 Mouse Monoclonal

AM3301
Annexin A6 Mouse Monoclonal

CM2811
Caveolin-1 Mouse Monoclonal

CM2831
Caveolin-1 (Tyr-14), phospho-specific Mouse Monoclonal

PP1491
PLCγ1 (Tyr-775), phospho-specific Rabbit Polyclonal

New Releases
AM0171
image
Annexin A5
Mouse Monoclonal
image
SP0281
image
nSMase3 (C-terminal region)
Rabbit Polyclonal
image
FM0221
image
Fibronectin
Mouse Monoclonal
image