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Technical Data Sheet

AM3301

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Annexin A6
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
67 kDa

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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Annexin A6 antibody at 1:250 (lane 1) or 1:1000 (lane 2).

Application
Dilution


ELISA
1:2000


IP
1:50


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.


Background References
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
Feinberg, J.M. et al. (1991) J Histochem. & Cytochem. 39:955.
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Immunogen
Clone (M330) was generated from a full length recombinant rat Annexin A6 protein.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 67 kDa* protein corresponding to the molecular mass of Annexin A6 on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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