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Technical Data Sheet

AX4325

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Arpc1b/p41-Arc (C-terminal region)
Blocking Peptide
Price
Size

$55
50 μg

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Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. The p41-Arc (Arpc1) subunit has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21.


Background References
Welch, M.D. et al. (1997) J Cell Biol. 138(2):375.
Vadlamudi, R.K. et al. (2004) EMBO Rep. 5(2):154.
Molli, P.R. et al. (2010) J Cell Biol. 190(1):101.
Sequence
Arpc1b/p41-Arc synthetic peptide corresponding to amino acid residues in the C-terminal region of human Arpc1b. This peptide sequence is highly conserved in rat, mouse, and chicken Arpc1b, and has some homology to the conserved region in isoform Arpc1a.
Buffer and Storage
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.
Store at –20°C. Stable for 1 year.
Specificity
The peptide is specifically recognized by anti-Arpc1b/p41-Arc (C-terminal region) antibody (AP4321) in ELISA, and has been shown to block the reactivity of AP4321 during Western blot. In addition, the peptide is recommended for use in blocking AP4321 reactivity in immunocytochemistry.
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AP4321
Arpc1b/p41-Arc (C-terminal region) Rabbit Polyclonal

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