ECM Logo Bar
Search :
Technical Data Sheet

CK6740

image
image
image
VE-Cadherin Phospho-Regulation
Antibody Sampler Kit
Price

$355

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
CP1981 Kishor, S.K. et al.(2015) Cardiovasc Res. 108(1):171-80 WB: HUVEC
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
CP1981 Adam, A.P. et al. (2010) J Biol Chem. 285(10):7045. WB: HDMECs
CP1981 Bowers, S. et al. (2010) 1188:143. Cell-Cell interactions
CP1981 Cain, R. J. et al. (2010) J Cell Biol. 188(6):863. WB: HUVECs
CP1981 Lo, CW et al. (2010) Cancer Res. 71(2):424. WB: HUVECs
CP2231 Bowers, S.L. et al. (2010) 1188:143. FB: negative control in Cell-Cell contact assay
Kit Summary
The VE-cadherin phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Tyr-685 relative to total expression of VE-cadherin. The kit includes a phospho-specific antibody to detect VE-cadherin Tyr-685, and mouse monoclonal and rabbit polyclonal antibodies to examine total VE-cadherin expression. The kit also includes secondary reagents for detection of primary antibodies in various applications.
image
image
Enlarge

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

image
Enlarge

Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).

image
Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

Background References
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Wallez Y. et al. (2007) Oncogene 26:1067.
Buffer and Storage
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
CK6260
E-Cadherin Phospho-Regulation Antibody Sampler Kit

CK6310
N-Cadherin Phospho-Regulation Antibody Sampler Kit

CM1681
E-Cadherin (Cytoplasmic) Mouse Monoclonal

CM1701
N-Cadherin (Cytoplasmic) Mouse Monoclonal

CP1801
N-Cadherin (Tyr-820), phospho-specific Rabbit Polyclonal

CP1901
N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit Polyclonal

CP1921
E-Cadherin (a.a. 774-786) Rabbit Polyclonal

CP1951
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal

CX1985
phospho-VE-Cadherin (Tyr-685) Blocking Peptide

CX2235
VE-Cadherin (a.a.770-781) Blocking Peptide

MS3011
Anti-Mouse Ig:DyLight® 488 Goat Polyclonal

MS3031
Anti-Mouse Ig:DyLight® 594 Goat Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

RS3241
Anti-Rabbit Ig Light-Chain Specific, unconjugated Mouse Monoclonal

New Releases
IK6750
image
Integrin α family
Antibody Sampler Kit
image
CM0261
image
CD99 (Extracellular region)
Mouse Monoclonal
image
CM0241
image
CD44 (Extracellular region)
Mouse Monoclonal
image