
Technical Data Sheet
CK6760



Cadherin Family (E-, N-, and P-)
Antibody Sampler Kit
Price
$395
Kit Components
Cat #
Description
Size
Applications
Species Reactivity
MW
(kDa)
(kDa)
CM1681
E-Cadherin (Cytoplasmic)
Mouse mAb
Mouse mAb
50 μl
WB, E, IP, ICC, IHC
Hu, Rt, Ms
120
CM5331
E-Cadherin (C-terminal fragment)
Mouse mAb
Mouse mAb
50 μl
WB, E
Hu, Rt, Ms
120
CP1921
E-Cadherin (a.a. 774-786)
Rabbit pAb
Rabbit pAb
50 μl
WB, E
Hu, Rt, Ms
120
CM1701
N-Cadherin (Cytoplasmic)
Mouse mAb
Mouse mAb
50 μl
WB, E, IP, ICC
Hu, Rt, Ms
130
CP1751
N-Cadherin (a.a. 811-824)
Rabbit pAb
Rabbit pAb
50 μl
WB, E, IP, ICC
Hu, Rt, Ms
130
CM5961
P-Cadherin (N-terminal region)
Mouse mAb
Mouse mAb
50 μl
WB, E, ICC
Hu, Rt, Ms
120
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish
Product References
CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1 cell
CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells
CP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules
CM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissue
CM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial Cells
CP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cells
CP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)
CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cells
CP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECs
Kit Summary
The cadherin family antibody sampler kit can be used to detect the expression level of E-cadherin, N-Cadherin, and P-cadherin. The kit includes high affinity mouse monoclonal and rabbit polyclonal antibodies to examine cadherin expression levels in western blot, ELISA, immunoprecipitation, and immunocytochemistry.


Enlarge
Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).

Enlarge
Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.

Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region.
Background References
Takeichi, M. (1988) Development 102:639.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Buffer and Storage
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

CP1801
N-Cadherin (Tyr-820), phospho-specific Rabbit Polyclonal
CP1851
Unphosphorylated N-Cadherin (Tyr-820) Rabbit Polyclonal
CP1901
N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit Polyclonal
CP1951
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal
CK6260
E-Cadherin Phospho-Regulation Antibody Sampler Kit
CK6310
N-Cadherin Phospho-Regulation Antibody Sampler Kit
CM1181
β-Catenin Mouse Monoclonal
CP1981
VE-Cadherin (Tyr-685), phospho-specific Rabbit Polyclonal
CP2231
VE-Cadherin (a.a.770-781) Rabbit Polyclonal
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