Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References

CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells

CP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules

CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1� cell

CM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissue

CM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial Cells

CP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cells

CP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)

CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cells

CP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECs

Kit Summary

The cadherin family antibody sampler kit can be used to detect the expression level of E-cadherin, N-Cadherin, and P-cadherin. The kit includes high affinity mouse monoclonal and rabbit polyclonal antibodies to examine cadherin expression levels in western blot, ELISA, immunoprecipitation, and immunocytochemistry.

Enlarge

Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).

Enlarge

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.

Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region.

Background References

Takeichi, M. (1988) Development 102:639.

Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.

Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.

Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

Buffer and Storage

Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

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