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Technical Data Sheet

CK7720

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Cytoskeletal Filament Labeling
Immunocytochemistry Kit
Price

$355

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
Kit Summary
The cytoskeletal filament kit can be used to label the major cytoskeletal elements in cells: actin filaments (Phalloidin:FITC), intermediate filaments (Vimentin), and microtubules (α-tubulin). The kit includes Phalloidin:FITC and Goat anti-mouse conjugated to DyLight® 594 for for dual labeling experiments.
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Immunocytochemical labeling of vimentin (VM4341; left), Phalloidin:FITC (PF7501; middle), and α-Tubulin (TM4111; right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 594.

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Background
The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Actin exists in two principal forms, globular, monomeric G-actin and filamentous, polymeric F-actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. Intermediate filament (IF) proteins are a diverse family with distinct expression patterns and functions. One major IF protein is vimentin, which forms filaments in mesenchymal, endothelial, and hematopoietic cells.

Background References
Wulf, E. et al. (1979) Proc. Nat. Acad. of Sci.(USA). 76:4498.
Faulstich, H. et al. (1983) Exp. Cell Res. 144:73.
Faulstich, H. et al. (1988) J. Muscle Res. Cell Motility. 9:370.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Sin, W.C. et al (1998) Mol Cell Biol. 18(11):6325.
Goto, H. et al. (1998) J Biol Chem. 273(19):11728.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.
Buffer and Storage
Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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