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Technical Data Sheet

CL7191

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Cdc42 Recombinant Fusion Protein
Lysate
Price
Size

$75
100 μl

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Enlarge

Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52 kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).

Application
Dilution


WB
10 μl/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

Rat Cdc42 full length protein (21 kDa) was generated as a fusion protein (52 kDa) from a pGEM expression vector system. The Cdc42 fusion protein is detected by mouse monoclonal anti-Cdc42 (Cat.#CM1521) and anti-Cdc42 (Cat.# CM4301).

Buffer and Storage
Cdc42 recombinant protein lysate is supplied at a concentration of 20 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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