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Technical Data Sheet

CM2991

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CaM Kinase IV (N-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
60 kDa

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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-CaM Kinase IV (N-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Hormones, growth factors and other cell signals cause increases in cytosolic Ca2+, which leads to cellular responses mediated by various Ca2+-binding proteins. Calmodulin (CaM) is a primary receptor for Ca2+ in cells. CaM functions as an allosteric activator of many enzymatic proteins including CaM kinases. CaM kinase IV is an important member of the CaM kinase family that has roles in neuronal long-term potentiation and memory, as well as T-cell receptor signaling. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaM kinase IV, releasing the autoinhibitory effect and thus activating the kinase. The activated CaMKIV further autophosphorylates itself at Thr-200 to render the kinase constitutively active. The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation.


Background References
Kang, H. et al. (2001) Cell 106:771.
Kasahara, J. et al. (2001) J. Biol. Chem. 276:24044.
Racioppi, L. & Means, A.R. (2008) Trends Immunol. 29(12):600.
Immunogen
Clone (M299) was generated from a recombinant sequence that included amino acids in the N-terminal region of human CaM Kinase IV. This sequence has high homology to rat and mouse CaM Kinase IV.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 60 kDa* protein corresponding to the molecular mass of CaM Kinase IV on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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