ECM Logo Bar
Search :
Technical Data Sheet

CM3561

image
image
image
δ1-Catenin (Tyr-228), phospho-specific
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
110 kDa

image
image
Enlarge

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

image
Enlarge

Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.

image
Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.


Background References
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Immunogen
Clone M356 was generated from a phospho-δ1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK6120
β-Catenin Phospho-Regulation Antibody Sampler Kit

CM3541
δ1-Catenin (a.a. 275-285) Mouse Monoclonal

CM3551
δ1-Catenin (Tyr-96), phospho-specific Mouse Monoclonal

CM3571
δ1-Catenin (Tyr-280), phospho-specific Mouse Monoclonal

CM3601
δ1-Catenin (Tyr-302), phospho-specific Mouse Monoclonal

CP3621
δ1-Catenin (Thr-916), phospho-specific Rabbit Polyclonal

New Releases
SP0281
image
nSMase3 (C-terminal region)
Rabbit Polyclonal
image
CM0301
image
CD99 (Extracellular region)
Mouse Monoclonal
image
CM0191
image
Cyclophilin B
Mouse Monoclonal
image