ECM Logo Bar
Search :
Technical Data Sheet

CM3821

image
image
image
Caspase-8 (C-terminal region)
Mouse Monoclonal IgG2a
Price
Size
Species Reactivity
MW

$245
100 μl
Hu
55 kDa

image
image
Enlarge

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.


Background References
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Immunogen
Clone (M382) was generated from a recombinant protein containing amino acid residues in the C-terminal region of human Caspase-8.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 55 kDa* protein corresponding to the molecular weight of Caspase-8 on SDS-PAGE immunoblots of human Jurkat, K562, and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK6360
Caspase Family Antibody Sampler Kit

CM3771
Caspase-3 (N-terminal region) Mouse Monoclonal

CM3781
Caspase-6 (C-terminal region) Mouse Monoclonal

CM3811
Caspase-7 (N-terminal region) Mouse Monoclonal

SM3591
Smac/DIABLO (C-terminal region) Mouse Monoclonal

TM3391
TRADD (C-terminal region) Mouse Monoclonal

New Releases
SP0281
image
nSMase3 (C-terminal region)
Rabbit Polyclonal
image
NM0161
image
Na+/K+ ATPase β3 (Extracellular)
Mouse Monoclonal
image
CM0241
image
CD44 (Extracellular region)
Mouse Monoclonal
image