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Technical Data Sheet

CM4301

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Cdc42
Mouse Monoclonal IgM
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
21 kDa

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Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52 kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).

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Enlarge

Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.


Background References
Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
Shinjo, K. et al. (1990) PNAS (USA) 87:9853.
Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.
Immunogen
Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
Buffer and Storage
Mouse monoclonal antibody purified with affinity chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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