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Technical Data Sheet

CM4891

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Caspase-1 (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
45 kDa

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Western blot analysis of Caspase-1 using anti-Caspase-1 monoclonal antibody (CM4891) at 1:1000 to probe blots containing human HeLa (A) and mouse NIH3T3 (B) lysates.

Application
Dilution


IHC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.


Background References
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Immunogen
Clone 14F468 was generated from a synthetic peptide corresponding to amino acids 371-390 (RKVRFSFEQPDGRAQMPTTE) in the C-terminal region of human caspase-1. This sequence has high homology to the conserved region in mouse and rat caspase-1.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.
Specificity
The antibody detects pro Caspase-1 at 45 kDa* and the cleaved forms of Caspase-1 that contain amino acid 371-390. The antibody can be used for western blot and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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