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Technical Data Sheet

CM4911

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Caspase-3 (p17 subunit)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
32 kDa

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Western blot analysis of Caspase-3 in multiple human tissues using CM4911 at 1:100. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively.

Application
Dilution


IHC
1:50


WB
1:250



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The caspases are a group of cysteine enzymes which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.


Background References
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Immunogen
Clone 31A1067 was developed against full-length recombinant human Caspase-3. The antibody recognizes an epitope in the large domain subunit (p17) of Caspase-3, and it will recognize pro Caspase-3 and the p17 cleavage fragment.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.
Specificity
The antibody detects pro Caspase-3 at 32 kDa* and the large subunit of the cleaved form of Caspase-3 at 14-21 kDa. The antibody can be used for western blot, immunohistochemistry and immuno-electron microscopy.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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