ECM Logo Bar
Search :
Technical Data Sheet

CM5011

image
image
image
CLASP1 (C-terminus)
Rat Monoclonal IgG2a
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck, F
161 kDa

image
image
Enlarge

Western blot of CLASP1 in mouse brain (lane 1) and rat PC12 cells (lane 2). The blots were probed with rat monoclonal CM5011 anti-CLASP1 (C-terminus) at a dilution of 1:500. Then detected using donkey anti-Rat IgG:HRP (RS3101).

Application
Dilution


ELISA
1:1000


ICC
1:100


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Maffini, S. et al. (2009) Curr Biol. 19(18):1566. WB,ICC: HeLa
image
Background
The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.


Background References
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
Al-Bassam, J. & Chang, F. (2011) Trends Cell Biol. 21(10):604.
Immunogen
Clone KT66 was generated from a GST fusion protein containing the C-terminus of mouse CLASP1. This sequence is highly conserved in human, rat, chicken, and fish CLASP1, and has low homology to CLASP2.
Buffer and Storage
Rat monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline and 0.05% sodium azide. Aliquot and Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 161 kDa* protein corresponding to the molecular mass of CLASP1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP1 by immunocytochemistry in HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AM5001
APC (C-terminal region) Rat Monoclonal

AM5021
APC (Central region) Rat Monoclonal

CM5051
CLASP2 (N-terminus) Rat Monoclonal

EM5041
EB1 (C-terminal region) Rat Monoclonal

EM5081
EB2 Rat Monoclonal

EM5101
EB3 Rat Monoclonal

New Releases
AM0211
image
Annexin A1
Mouse Monoclonal
image
SP0281
image
nSMase3 (C-terminal region)
Rabbit Polyclonal
image
AM0171
image
Annexin A5
Mouse Monoclonal
image