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Technical Data Sheet

CP1751

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N-Cadherin (a.a. 811-824)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
130 kDa

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Enlarge

Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).

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Enlarge

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.

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Application
Dilution


ELISA
1:2000


ICC
1:200


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1 cell
Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cells
Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)
Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECs
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Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.


Background References
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Immunogen
unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids 811 to 824 from human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using N-cadherin (a.a. 811-824) peptide. The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CM1681
E-Cadherin (Cytoplasmic) Mouse Monoclonal

CM1701
N-Cadherin (Cytoplasmic) Mouse Monoclonal

CP1801
N-Cadherin (Tyr-820), phospho-specific Rabbit Polyclonal

CP1851
Unphosphorylated N-Cadherin (Tyr-820) Rabbit Polyclonal

CP1901
N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit Polyclonal

CP1951
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal

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