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Technical Data Sheet

CP1921

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E-Cadherin (a.a. 774-786)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
120 kDa

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Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells
SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules
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Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.


Background References
Takeichi, M. (1988) Development 102:639.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Immunogen
E-cadherin synthetic peptide corresponding to amino acids in the C-terminal region in human E-cadherin. This sequence is conserved in rat and mouse E-cadherin, and has low homology to other cadherin family members.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using E-cadherin (a.a. 774-786) peptide. In western blots, the antibody detects a 120 kDa band corresponding to E-cadherin in human A431 cells, and does not detect VE-cadherin or N-Cadherin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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