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Technical Data Sheet

CP2581

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Coronin-1B (C-terminus)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
60 kDa

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Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Coronin-1B (C-terminal region) (lanes 1 & 2) and anti-Coronin-1B (Ser-2) (lanes 3 & 4).

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Immunocytochemical labeling of coronin-1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Coronin-1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2585 or CX2625).

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Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.


Background References
Foger, N. et al. (2006) Science 313:839.
Uetrecht, A.C. & Bear, J.E. (2006) Trends Cell Biol. 16(8):421.
Cai, L. et al. (2007) Cell 128:915.
Immunogen
Coronin-1B (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids at the C-terminus of human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B, and less than 50% homology to similar regions in other coronins.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using Coronin-1B (C-terminus) peptide (without carrier). The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 and HeLa, mouse brain, and rabbit spleen fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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