ECM Logo Bar
Search :
Technical Data Sheet

CX1985

image
image
image
phospho-VE-Cadherin (Tyr-685)
Blocking Peptide
Price
Size

$55
50 μg

image
Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.


Background References
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Wallez Y. et al. (2007) Oncogene 26:1067.
Sequence
Phospho-VE-Cadherin (Tyr-685) synthetic peptide contains amino acids surrounding tyrosine 685 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse VE-cadherin, but is not conserved in other cadherins.
Buffer and Storage
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The peptide is specifically recognized by anti-VE-Cadherin (Tyr-685) antibody (CP1981) in ELISA, and has been shown to block the reactivity of CP1981 during Western blot. In addition, the peptide is recommended for use in blocking CP1981 reactivity in immunocytochemistry.
imageRelated Products
CM1681
E-Cadherin (Cytoplasmic) Mouse Monoclonal

CP1751
N-Cadherin (a.a. 811-824) Rabbit Polyclonal

CP1801
N-Cadherin (Tyr-820), phospho-specific Rabbit Polyclonal

CP1921
E-Cadherin (a.a. 774-786) Rabbit Polyclonal

CP1981
VE-Cadherin (Tyr-685), phospho-specific Rabbit Polyclonal

New Releases
CM0111
image
CD59 (glycoprotein)
Mouse Monoclonal
image
IK6700
image
Integrin β family
Antibody Sampler Kit
image
FM0311
image
FXYD5/Dysadherin (Extracellular region)
Mouse Monoclonal
image