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Technical Data Sheet

CX2235

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VE-Cadherin (a.a.770-781)
Blocking Peptide
Price
Size

$55
50 μg

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Western blot image of human umbilical vein endothelial cells. The blots were probed with rabbit polyclonal anti-VE-cadherin (a.a. 770-781) at 1:1000 (lane 1) and 1:4000 (lane 2). In addition, the antibody was used in the presence (lane 4) or absence (lane 3) of blocking peptide.

Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.


Background References
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Wallez Y. et al. (2007) Oncogene 26:1067.
Sequence
VE-Cadherin synthetic peptide corresponds to amino acids 770 to 781 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse, and has less than 50% homology with other cadherins.
Buffer and Storage
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.
Store at –20°C. Stable for 1 year.
Specificity
This peptide is specifically recognized by VE-cadherin (a.a. 770-781) antibody (CP2231) in ELISA, and has been shown to block the reactivity of CP2231 in Western blot and is recommended for blocking in immunocytochemistry.
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CP1951
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal

CP1981
VE-Cadherin (Tyr-685), phospho-specific Rabbit Polyclonal

CP2231
VE-Cadherin (a.a.770-781) Rabbit Polyclonal

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