ECM Logo Bar
Search :
Technical Data Sheet

FM0131

image
image
image
Fibronectin (Cell/Heparin2 Binding region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
250 kDa

image
image
Enlarge

Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.

image
Enlarge

Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M013). The antibody was detected using goat anti-mouse Ig DyLight® 594.

image
Application
Dilution


ELISA
1:2000


ICC
1:50


IP
1:50


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.


Background References
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Zollinger AJ & Smith ML (2017) Matrix Biol. 60-61:27.
Immunogen
Clone (M013) was generated from a proprietary antigen related to native human fibronectin from the A549 lung carcinoma cell line.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M013 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody does not detect the denatured form of fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK7720
Cytoskeletal Filament Labeling Immunocytochemistry Kit

FM0141
Fibronectin Mouse Monoclonal

FM0221
Fibronectin Mouse Monoclonal

IK6700
Integrin β family Antibody Sampler Kit

IK6750
Integrin α family Antibody Sampler Kit

LM5971
Laminin γ1 subunit (Domain IV) Mouse Monoclonal

New Releases
CM0301
image
CD99 (Extracellular region)
Mouse Monoclonal
image
IK6700
image
Integrin β family
Antibody Sampler Kit
image
IM0061
image
Integrin β1 (Extracellular region)
Mouse Monoclonal
image