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Technical Data Sheet

GM3421

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GM130 (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
130 kDa

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Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.

Application
Dilution


ELISA
1:1000


ICC
1:50


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Martín-Acebes, M.A. et al. (2014) J virology 88(20): 12041. Data not shown
Martın-Acebes, M.A. et al. (2011) PLoS ONE 6(9): e24970. ICC: monkey Vero cell
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Background
Golgi Matrix Protein (GM130) is a peripheral cytoplasmic protein that is bound to Golgi membranes. It maintains cis-Golgi structure, and it regulates the disassembly and reassembly of the Golgi complex during mitosis. GM130 may also be important during docking and fusion of coatomer (COPI) coated vesicles to the Golgi membrane. The carboxy-terminal domain of GM130 is highly homologous to the human auto-antigen, golgin-95. GM130 interacts in a GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes. It has also been implicated in the activation of Ste-kinase, YSK1, during the golgi reorganization that occurs along with cell migration.


Background References
Warren, G. (1993) Annual Rev Biochem 62:323.
Nakamura, N. et al. (1995) J Cell Biol 131:1715.
Weide, T. et al. (2001) EMBO Rep 2:336.
Immunogen
Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.
Buffer and Storage
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of GM130 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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