Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Kit Summary

The Integrin β family antibody sampler kit can be used to detect the expression level of Integrin β1, Integrin β3, and Integrin β4. The kit includes high affinity mouse monoclonal antibodies to examine Integrin β expression levels in western blot, immunoprecipitation, and immunocytochemistry.

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Western blot analysis of human umbilical vein endothelial cells (HUVEC). The blots were probed with mouse monoclonal anti-integrin β3 (IM5811) at 1:250 (lane 1) and 1:1000 (lane 2).

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Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M006). The antibody was detected using goat anti-mouse DyLight® 594.

Background

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis. Integrin α6β4 receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the integrin-β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. The integrin β3 subunit associates with integrin α2 in platelets and with integrin αV subunit to form the vitronectin receptor in endothelial cells. This receptor mediates endothelial cell adhesion to vitronectin, fibrinogen, von Willebrand factor, thrombospondin, laminin, and fibronectin.

Background References

Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.

Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.

Wang, L. et al. (2012) J Cell Physiol. 227(2):474.

Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.

Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.

Buffer and Storage

Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

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