ECM Logo Bar
Search :
Technical Data Sheet

IK6750

image
image
image
Integrin α family
Antibody Sampler Kit
Price

$405

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
Kit Summary
The Integrin α family antibody sampler kit can be used to detect the expression level of Integrin α2, Integrin α5, Integrin αL, and Integrin αV. The kit includes high affinity mouse monoclonal antibodies to examine Integrin α expression levels in western blot, immunoprecipitation, and immunocytochemistry.
image
image
Enlarge

Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-integrin αL (IM5941) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).

image
Enlarge

Western blot analysis of human MDA-MB-231 (lane 1) and A431 (lane 2) cells. The blot was probed with mouse monoclonal anti-Integrin α2 (IM5831) at 1:1000 dilution.

image
Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members, while integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor in endothelial cells.

Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
Buffer and Storage
Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.The secondary reagent is supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
IK6270
Integrin β4 Phospho-Regulation Antibody Sampler Kit

IM1261
Integrin β4 (Cytoplasmic region) M126 Mouse Monoclonal

IM5811
Integrin β3 (Extracellular region) Mouse Monoclonal

IM5821
Integrin β1 (Extracellular region) Mouse Monoclonal

MS3011
Anti-Mouse Ig:DyLight® 488 Goat Polyclonal

MS3031
Anti-Mouse Ig:DyLight® 594 Goat Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3251
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse Monoclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

New Releases
AM0211
image
Annexin A1
Mouse Monoclonal
image
LM0231
image
L1CAM (Extracellular)
Mouse Monoclonal
image
IK6750
image
Integrin α family
Antibody Sampler Kit
image