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Technical Data Sheet

IM0321

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Integrin β1 (Extracellular region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
130 kDa

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Western blot analysis of integrin β1 expression in native human cells and tissues: A549 (lane 1) LNCaP (lane 2), MDA-MB-231 (lane 3) breast (lane 4), lung (lane 5), and skin (lane 6). The blot was probed with mouse monoclonal anti-Integrin β1 (IM0321) at 1:1000 dilution.

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Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (IM0321). The antibody was detected using goat anti-mouse Ig DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Immunogen
Clone M032 was generated from a proprietary antigen related to the extracellular region of human integrin β1 expressed in A431 epidermoid carcinoma cell line.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M032 detects a 120-150 kDa* band corresponding to Integrin β1 on SDS-PAGE immunoblots of "Native" human A431, A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues. The antibody weakly detects the denatured form of Integrin β1. Clone M032 can be used in western blot, immunocytochemistry, and ELISA.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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