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Technical Data Sheet

IM4781

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IKKγ/NEMO
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
50 kDa

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Western blot analysis of IKKγ in human Jurkat cells. The blot was probed with mouse monoclonal IKKγ (IM4781) at a dilution of 1:250.

Application
Dilution


FC
1:500


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
NF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKKα, IKKβ, and IKKγ. IKKα and IKKβ are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKKβ, not IKKα, is the target of proinflammatory stimuli. Targeted disruption of IKKβ gene in mice also results in extensive liver damage from apotopsis and death during embryo development.


Background References
Delhase, M. et al. (1999) Science 284:309.
Hu, Y. et al. (1999) Science 284:316.
Takada, K. et al. (1999) Science 284:313.
Immunogen
Clone 46B844 was generated from recombinant full length human IKKγ. This sequence is well conserved in rat and mouse IKKγ.
Buffer and Storage
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Specificity
This antibody detects IKKγ at 50 kDa* on SDS-PAGE immunoblots of human Jurkat cells. The antibody can be used for western blot and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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