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Technical Data Sheet

LP1831

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LIMK1 (C-terminus)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
72 kDa

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Enlarge

Western blot image of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-term.) (lanes 1 & 2) and anti-LIMK1 (Thr-508) (lanes 3 & 4).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.


Background References
Okano, I. et al. (1995) J. Biol. Chem. 270:31321.
Edwards, D. C. et al. (1999) Nat. Cell Biol. 1:253.
Kobayashi, M. et al. (2006) EMBOJ 25:713.
Immunogen
LIMK1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponding to amino acids at the C-terminus of human LIMK1. This sequence is conserved in rat and mouse LIMK1, and is not found in LIMK2.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using LIMK1 (C-terminus) peptide (without carrier). The antibody detects a 72 kDa* protein corresponding to the molecular mass of LIMK1 on SDS-PAGE immunoblots of mouse recombinant LIMK1.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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