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Technical Data Sheet

LP2431

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LIMK1 (Ser-323)[LIMK2 (Ser-314)], phospho-specific
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
72 kDa

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Enlarge

Western blot image of human A431 cells untreated (lanes 1 & 3) or treated (lanes 2 & 4)with calyculin A (100 nM for 30 min) . The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or anti-LIMK1 (Ser-323) (lanes 3 & 4).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.


Background References
Okano, I. et al. (1995) J. Biol. Chem. 270:31321.
Edwards, D. C. et al. (1999) Nat. Cell Biol. 1:253.
Kobayashi, M. et al. (2006) EMBOJ 25:713.
Immunogen
LIMK1 (Ser-323) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 323 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Ser-314 in human LIMK2.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was cross-adsorbed to unphosphorylated LIMK1 (Ser-323) peptide then affinity purified using LIMK1 (Ser-323) peptide (without carrier). The antibody detects a protein with the same mobility as anti-LIMK1 (C-terminus, LP1831) in human A431 cells treated with calyculin A. This band is weak in control cells and is not detected after lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
LP1831
LIMK1 (C-terminus) Rabbit Polyclonal

LP1891
LIMK1 (Thr-508)[LIMK2 (Thr-505], phospho-specific Rabbit Polyclonal

LX2435
phospho-LIMK1 (Ser-323) Blocking Peptide

LX2445
unphosphorylated LIMK1 (Ser-323) Blocking Peptide

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