ECM Logo Bar
Search :
Technical Data Sheet

MK6370

image
image
image
Myosin IIA Heavy Chain Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
MP3831 Guilbert, A. et al. (2013) Eur J Cancer. 49(17):3694. WB: MDA-MB-231 cells
MP3831 Sanborn, K.B. et al. (2013) Blood 118(22):5862. WB: human YTS cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
Kit Summary
The non-muscle myosin IIA heavy chain (NMHC-IIA) phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of NMHC-IIA at Ser-1803, Ser-1916, and Ser-1943 relative to the total expression level of NMHC-IIA. The kit includes rabbit polyclonal phospho-specific antibodies to Ser-1803, Ser-1916, and Ser-1943, a rabbit polyclonal antibody to detect total NMHC-IIA, as well as a secondary regent for detection of antibodies in western blot, ELISA, or immunocytochemistry.
image
image
Enlarge

Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).

image
Enlarge

Immunocytochemical labeling of myosin IIA heavy chain phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943) antibody (MP3831), then detected using appropriate secondary antibody (Bottom, Red). This labeling is compared to F-actin staining (Bottom, Green) and to secondary only (Top). (Image provided by Dr. Gianluca Gallo at Drexel University).

image
Background
Non-muscle myosin II is an actin-based motor protein essential to cell motility, division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, which activates myosin II motor activity and promotes filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. Several kinases phosphorylation NMHC-II isoforms directly. TRPM7 phosphorylates Thr-1800, Ser-1803, and Ser-1808, which reduces NMHC-IIA incorporation into the actin cytoskeleton. PKC phosphorylates NMHC-IIA on Ser-1916 and NMHC-IIB on multiple tailpiece serines leading to inhibition of filament assembly. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 and increases disassembly of NMHC-IIA filaments.

Background References
Murakami, N. et al. (1998) Biochemistry 37:1989.
Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18:3144.
Conti, M.A. & Adelstein, R.S. (2008) J Cell Sci. 121:11.
Breckenridge, M.T. et al. (2009) Mol Biol Cell 20:338.
Dulyaninova, N. & Bresnick, A. (2013) BioArchitect. 3(4):1.
Buffer and Storage
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
MK6370
Myosin IIA Heavy Chain Phospho-Regulation Antibody Sampler Kit

MM3441
Myosin Light Chain (MLC20) Mouse Monoclonal

MP3461
Myosin Light Chain (Ser-1), phospho-specific Rabbit Polyclonal

MX3795
Myosin IIA Heavy Chain (a.a. 1936-1950) Blocking Peptide

RP1361
RhoA (Ser-188), phospho-specific Rabbit Polyclonal

MX3835
phospho-Myosin IIA Heavy Chain (Ser-1943) Blocking Peptide

MP4201
Myosin Light Chain (a.a. 11-22) Rabbit Polyclonal

MP4221
Myosin Light Chain (Ser-19), phospho-specific Rabbit Polyclonal

MS3001
Anti-Mouse Ig:HRP Donkey Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3241
Anti-Rabbit Ig Light-Chain Specific, unconjugated Mouse Monoclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

New Releases
AM0211
image
Annexin A1
Mouse Monoclonal
image
FM0311
image
FXYD5/Dysadherin (Extracellular region)
Mouse Monoclonal
image
FM0131
image
Fibronectin (Cell/Heparin2 Binding region)
Mouse Monoclonal
image