
Technical Data Sheet
MK7640



Microtubule Labeling
Immunocytochemistry Kit
Price
$355
Kit Components
Cat #
Description
Size
Applications
Species Reactivity
ICC Dilution
TM4111
α-Tubulin (C-terminus)
Mouse mAb
Mouse mAb
50 μl
WB, E, IP, ICC, IHC
Hu, Rt, Ms
1:200
TM1541
β-Tubulin
Mouse mAb
Mouse mAb
50 μl
WB, E, ICC
Hu, Rt, Ms
1:200
TP1691
βIII-Tubulin (C-terminus)
Rabbit pAb
Rabbit pAb
50 μl
WB, E, ICC, IHC
Hu, Rt, Ms
1:200
MS3011
Anti-Mouse Ig:DyLight® 488
Goat pAb
Goat pAb
100 μl
ICC, IHC
Ms
1:200
RS3271
Anti-Rabbit Ig:DyLight® 594
Goat pAb
Goat pAb
100 μl
ICC, IHC
Rb
1:200
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish
Product References
TP1691 Sitaras, N. et al. (2015) Am J Pathol. 185(2):581 IF: mouse retinal ganglion cells
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
TP1691 Saphieha, P. et al. (2012) Nutr Diabetes. 2:36. ICC: mouse retina cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells
Kit Summary
The microtubule lableing kit can be used for immunocytochemical labeling of tubulin. The kit includes monoclonal antibodies to α- and βI-tubulin, and a rabbit polyclonal antibody to βIII-tubulin. The kit also includes goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.


Enlarge
Immunocytochemical labeling of Tubulin isoforms. In rat A7r5 cells, microtubules were labeled using anti-βI-Tubulin (TM1541) (left) and anti-α-tubulin (TM4111) (right). In NGF-differentiated rat PC12 cells, microtubules were labeled using anti-βIII-Tubulin (TP1691) (middle). The antibodies were detected using the appropriate secondary antibody conjugated to DyLight® 488 or 594.

Background
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate multiple residues, and phosphorylation of Ser-165 by PKC has been implicated in promoting cell motility.
Background References
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.
Buffer and Storage
Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

MS3031
Anti-Mouse Ig:DyLight® 594 Goat Polyclonal
PF7501
Phalloidin:FITC Reagent
PF7551
Phalloidin:TRITC Reagent
RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal
AK6060
Actin & Tubulin Antibody Sampler Kit
CK7720
Cytoskeletal Filament Labeling Immunocytochemistry Kit
TP1721
β-Tubulin (Ser-172), phospho-specific Rabbit Polyclonal
TP1781
β-Tubulin (a.a. 168-177) Rabbit Polyclonal
TP1811
unphosphorylated βIII-Tubulin (Ser-444) Rabbit Polyclonal
MK7640
Microtubule Labeling Immunocytochemistry Kit
VM4341
Vimentin Mouse Monoclonal
New Releases
AM0181


Annexin A1
Mouse Monoclonal
Mouse Monoclonal

CM0151


CD59 (glycoprotein)
Mouse Monoclonal
Mouse Monoclonal

CM0101


CD44 (Extracellular region)
Mouse Monoclonal
Mouse Monoclonal

