ECM Logo Bar
Search :
Technical Data Sheet

SM1351

image
image
image
Stat1 (Tyr-701), phospho-specific
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
91 kDa

image
image
Enlarge

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.


Background References
Fu, X.Y. et al. (1993) Cell 74:1135.
Gouilleux, F. et al. (1994) EMBO J. 13:4361.
Darnell, J.E. (1997) Science 277:1630.
Immunogen
Clone (M135) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 701 of human Stat1. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
EK6160
EGFR Phospho-Regulation Antibody Sampler Kit

MK6050
MAP Kinase Activation Antibody Sampler Kit

PM1381
p38α MAP Kinase (C-terminal) M138 Mouse Monoclonal

PM1391
p38 MAP Kinase (Thr-180/Tyr-182), phospho-specific Mouse Monoclonal

SM1481
Stat5 (Tyr-694), phospho-specific Mouse Monoclonal

SM2491
Stat1 Mouse Monoclonal

SM2511
Stat5 (C-terminal region) Mouse Monoclonal

SM2631
Stat3 (N-terminal region) Mouse Monoclonal

New Releases
NM0201
image
Na+/K+ ATPase β3
Mouse Monoclonal
image
IM0321
image
Integrin β1 (Extracellular region)
Mouse Monoclonal
image
CM0021
image
CD55 (Extracellular region)
Mouse Monoclonal
image