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Technical Data Sheet

SM2631

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Stat3 (N-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
86 kDa

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Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Application
Dilution


ELISA
1:2000


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.


Background References
Wen, Z. et al. (1995) Cell 82:241.
Darnell, J.E. (1997) Science 277:1630.
Biethahn, S. et al. (1999) Exp. Hematol. 27:885.
Immunogen
Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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