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Technical Data Sheet

SM3651

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Synapsin I (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
80 kDa

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Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Synapsin I (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).

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Immunocytochemical labeling of Synapsin I in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Synapsin I (C-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

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Application
Dilution


ELISA
1:2000


ICC
1:50


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Chanaday, N. L. et al. (2015) Mol Neurobiol 51(3): 1353. WB: rat synaptosomes
Geng, D. et al. (2013) Neurochem Int. 63(4):283. IHC: human hippocampal neurons
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Background
Synapsins are important peripheral membrane proteins involved in synaptic vesicle release during neurotransmission between neurons. Several synapsin variants are generated by alternative splicing of the mRNA from three different genes: synapsin I, II, and III. Synapsins bind to synaptic vesicles via their conserved N-terminal domains. Regulation of synapsins may occur through PKA and CAM kinase I phosphorylation. This regulation occurs concomitantly with membrane excitation and neurotransmitter release into the synaptic cleft. Synapsins bundle actin filaments and interact with multiple calcium binding proteins. Thus, synapsins may crosslink vesicles or other proteins to actin filaments at the presynaptic terminal. In addition, synapsins may interact with each other, since synapsin III has been shown to form heteromultimers with both synapsin I and synapsin II. All synapsins contain a central ATP binding domain and variable C-terminal domains that facilitate distinct regulatory functions.


Background References
Südhof, T.C. et al. (1989) Science. 245(4925):1474.
Südhof, T.C. (1990) J Biol Chem. 265(14):7849.
Fdez, E. & Hilfiker, S. (2006) Brain Cell Biol. 35(2-3):107.
Immunogen
Clone M365 was generated from a sequence corresponding to amino acids in the C-terminal region of mouse synapsin I. This sequence has high homology to human and rat synpasin I.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects an 80 kDa* protein corresponding to the molecular mass of Synapsin I on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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