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Technical Data Sheet

SM3711

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Syntaphilin (N-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
70 kDa

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Western blot of adult mouse brain lysate. The blot was probed with mouse monoclonal anti-Syntaphilin (N-terminal region) antibody at 1:250 (lane 1), 1:1000 (lane 2), or 1:2000 (lane 3).

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Synaptic vesicles are organelles situated at the distal terminus of the presynaptic neuron. The exocytosis of these vesicles requires docking at the plasma membrane, priming, and fusion. Fusion is mediated by a complex consisting of membrane components of both the synaptic vesicle and the synaptic plasma membrane. The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25kDa). Syntaxin-1 is a key component of the synaptic vesicle docking/fusion machinery which forms the SNARE complex with SNAP-25 and synaptobrevin. Syntaphilin is a brain-specific membrane-associated protein that can inhibit SNARE complex formation by binding free syntaxin-1.


Background References
Lao, G. et al. (2000) Neuron 25:191.
Chen, Y.M. et al. (2009) J Neurosci. 29(30):9429.
Immunogen
Clone M371 was generated from a sequence corresponding to amino acids in the N-terminal region of human syntaphilin. This sequence has high homology to mouse and rat syntaphilin.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 70 kDa* protein corresponding to the molecular mass of Syntaphilin on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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