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Technical Data Sheet

SM3731

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Syk (Central region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
72 kDa

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Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-Syk (Central region) antibody at 1:250.

Application
Dilution


ELISA
1:1000


WB
1:250



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all hematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igα, TCRζ, CD3ε, FcεRIβ, and FceRIγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains, thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cγ1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways.


Background References
Costello, P.S. et al. (1996) Oncogene. 13(12):2595.
Latour, S. et al. (2001) Curr Opin Immunol. 13:299.
Immunogen
Clone M373 was generated from a sequence corresponding to amino acids in the central region of human Syk. This sequence has high homology to mouse and rat Syk.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 72 kDa* protein corresponding to the molecular mass of Syk on SDS-PAGE immunoblots of adult mouse spleen.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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