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Technical Data Sheet

SP1221

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Semaphorin-3A (Central region)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
95 kDa

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Enlarge

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

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Enlarge

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

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Application
Dilution


ELISA
1:2000


ICC
1:100


IHC
1:300


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
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Background
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.


Background References
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Luo, Y. et al. (1993) Cell 75:217.
Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Immunogen
Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Sema3A. The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using Semaphorin 3A (Central region) peptide (without carrier). The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A and neonatal rat brain lysates.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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SP1241
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SP1401
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SX1225
Semaphorin-3A (Central region) Blocking Peptide

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