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Technical Data Sheet

TM0051

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Trop-2/TACSTD2 (Extracellular region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
60 kDa

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Western blot analysis of human A431 (lanes 1 & 2), MDA-MB-231 (lanes 3 & 4), and MCF-7 (lanes 5 & 6) cell lysates under native (lanes 1, 3 & 5) and denatured (lanes 2, 4 & 6) conditions. The blots were probed with mouse monoclonal anti-Trop-2 (TM0051) at 1:1000 (lanes 1-6).

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Immunocytochemical labeling of Trop-2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Trop-2 (clone M005). The antibody was detected using goat anti-mouse DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:100


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Trop-2 (TACSTD2) is a transmembrane glycoprotein found on invasive trophoblast cells and in several epithelial type cancer cells. Trop-2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site. This glycoprotein functions in many signaling pathways including interaction of its extracellular domain with integrin β1 to regulate FAK signaling, interaction of its transmembrane domain with claudin 1 and claudin 7 during tight junction formation, and regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway. These Trop-2 functions may be important during tumor proliferation, metastasis and invasion.


Background References
McDougall, AR et al. (2015) Dev Dyn. 244:99
Shvartsur A & Bonavida B (2015) Genes Cancer 6(3-4):84
Immunogen
Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M005 detects a 50-65 kDa* band corresponding to the molecular mass of Trop-2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured Trop-2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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