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Technical Data Sheet

TM1541

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β-Tubulin
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$195
100 μl
Hu, Rt, Ms
50 kDa

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Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

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Immunocytochemical labeling in C2C12 cells using anti-β-Tubulin (TM1541) monoclonal antibody and anti-β-Tubulin (Ser-172) polyclonal antibody. The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of phospho-β-Tubulin (Ser-172) peptide (TX1725).

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Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells
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Background
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.


Background References
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Immunogen
Clone (M154) was generated from purified porcine brain tubulin.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 50 kDa* protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin, mouse brain tissue, rat PC12 cells, and human A431 and SH-SY5Y cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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TP1691
βIII-Tubulin (C-terminus) Rabbit Polyclonal

TP1721
β-Tubulin (Ser-172), phospho-specific Rabbit Polyclonal

TP1781
β-Tubulin (a.a. 168-177) Rabbit Polyclonal

TP1811
unphosphorylated βIII-Tubulin (Ser-444) Rabbit Polyclonal

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