ECM Logo Bar
Search :
Technical Data Sheet

TM3391

image
image
image
TRADD (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
34 kDa

image
image
Enlarge

Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal TRADD (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).

image
Enlarge

Immunocytochemical labeling of TRADD in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with mouse monoclonal TRADD (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

image
Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Tumor necrosis factor (TNF) activates TNF receptor-1 (TNFR1), leading to diverse biological activities in many cell types. TNFR1 contains an intracellular death domain that is required for signaling related to antiviral activity, programmed cell death, and NF-κB activation. TNFR1-associated death domain protein (TRADD) was isolated in a screen to identify proteins that interact with the death domain of TNFR1. TRADD is a 34-kDa protein that contains a 111-amino acid death domain with sequence similarity to TNFR1. This domain mediates oligomerization of TRADD and interaction with TNFR1. TRADD mRNA is expressed ubiquitously, and overexpression of TRADD causes TNF-induced apoptosis and activation of NF-κB. These responses require the TRADD death domain. In addition to TNF signaling, TRADD N-terminus can interact with Stat1 in response to IFNγ, and this interaction can negatively regulate Stat activity.


Background References
Hsu, H. et al. (1995) Cell 81:495.
Wesemann, D.R. et al. (2004) Nat. Immun. 5:199.
Immunogen
Clone M339 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human TRADD. This sequence has high homology to similar regions in rat and mouse TRADD.
Buffer and Storage
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 34 kDa* protein corresponding to the apparent molecular mass of TRADD on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
IP1031
IκBα (Tyr-42), phospho-specific Rabbit Polyclonal

IP1041
IκBα (Tyr-305), phospho-specific Rabbit Polyclonal

IP1861
IκBα (C-terminus) Rabbit Polyclonal

SK6030
Stat Phospho-Regulation Antibody Sampler Kit

SM1351
Stat1 (Tyr-701), phospho-specific Mouse Monoclonal

SM2491
Stat1 Mouse Monoclonal

New Releases
AM0171
image
Annexin A5
Mouse Monoclonal
image
CM0341
image
CD63 (Extracellular region)
Mouse Monoclonal
image
FM0291
image
FXYD5/Dysadherin (Extracellular region)
Mouse Monoclonal
image