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Technical Data Sheet

TP3881

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Themis (N-terminal region)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
72 kDa

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Enlarge

Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (TX3885) (lanes 4 & 8).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
During positive selection, thymocytes transition through a stage during which T cell antigen receptor (TCR) signaling controls CD4-versus-CD8 lineage selection and maturation. Thymocyte-expressed molecule involved in selection (themis) is critically required for this thymocyte selection. Themis has been identified as several molecules: Grb2-associating protein (GASP), signaling phosphoprotein specific for T cells (SPoT), TSEPA, and thylex. Themis is a cytosolic protein with no known functional motifs. Its expression is observed primarily in T cells, especially immature CD4/CD8 double positive thymocytes. Themis-deficient thymocytes have defective positive selection, and fewer numbers of mature thymocytes. These themis-induced effects may be due to defective TCR signaling since themis constitutively associates with the TCR adaptor Grb2, and themis is phosphorylated quickly after TCR stimulation. In addition, themis is required for optimal TCR-driven calcium mobilization and activation of the MAP kinase Erk. Thus, themis may be an important adaptor protein in TCR signaling.


Background References
Fu, G., et al. (2009) Nat Immunol. 10(8):848.
Johnson, A.L., et al. (2009) Nat Immunol. 10(8):831.
Kakugawa, K., et al. (2009) Mol Cell Biol. 29(18):5128.
Lesourne, R., et al. (2009) Nat Immunol. 10(8):840.
Patrick M.S., et al. (2009) Proc Natl Acad Sci U S A. 106(38):16345.
Immunogen
Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using themis (N-terminal region) peptide (without carrier). The antibody detects a 72 kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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TX3885
Themis (N-terminal region) Blocking Peptide

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