ECM Logo Bar
Search :
Technical Data Sheet

TP4171

image
image
image
Talin (Ser-425), phospho-specific
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms, Ck, F
240 kDa

image
image
Enlarge

Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.

image
Enlarge

Immunocytochemical labeling of Talin phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Talin (Ser-425) antibody (TP4171), then the antibody was detected using appropriate secondary antibody (Green). This labeling is compared to F-actin staining (Red, Left). (Image provided by Dr. Gianluca Gallo at Drexel University).

image
Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Jin, J.K. et al. (2015) Oncogene. 34(14): 1811. WB: metastatic PCa cells, PC3-MM2, shRNA
image
Background
Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation, and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration.


Background References
Ratnikov, B. et al. (2005) J. Cell Sci. 118:4921.
Huang, C. et al. (2009) Nat Cell Biol. 11(5):624.
Moser, M. et al. (2009) Science. 324(5929):895.
Immunogen
Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6060
Actin & Tubulin Antibody Sampler Kit

IK6270
Integrin β4 Phospho-Regulation Antibody Sampler Kit

PK6070
Paxillin Phospho-Regulation Antibody Sampler Kit

TM4081
Talin (Rod domain) Mouse Monoclonal

TX4175
phospho-Talin (Ser-425) Blocking Peptide

TX4185
Talin (a.a. 420-429) Blocking Peptide

New Releases
AM0181
image
Annexin A1
Mouse Monoclonal
image
CM0241
image
CD44 (Extracellular region)
Mouse Monoclonal
image
AM0171
image
Annexin A5
Mouse Monoclonal
image