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Technical Data Sheet

VM4341

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Vimentin
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck
58 kDa

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Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Application
Dilution


ELISA
1:1000


ICC
1:100


WB
1:200



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.


Background References
Goto, H. et al. (1998) J Biol Chem. 273(19):11728.
Sin, W.C. et al (1998) Mol Cell Biol. 18(11):6325.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Immunogen
Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68). In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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