ECM Logo Bar
Search :
Technical Data Sheet

VP2481

image
image
image
Vav (a.a. 165-174) [conserved site]
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
100 kDa

image
image
Enlarge

Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).

image
Enlarge

Immunocytochemical labeling of Vav in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Vav (a.a. 165-174), and detected using appropriate secondary antibody conjugated to Cy3.

image
Application
Dilution


ELISA
1:2000


ICC
1:50


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.


Background References
Schuebel, K.E. et al. (1996) Oncogene 13:363.
Aghazadeh, B. (2000) Cell 102:625.
Wilsbacher, J.L. et al. (2006) Cell Comm. Signal. 4:5.
Immunogen
Vav2 (a.a. 165-174) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using Vav2 (a.a. 165-174) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav2 on SDS-PAGE immunoblots of human A431 cells and detects several Vav isoforms in Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
VP2521
Vav2 (a.a. 309-322) Rabbit Polyclonal

VP2561
Vav2 (Tyr-142)[conserved site], phospho-specific Rabbit Polyclonal

VP2641
Vav2 (Tyr-172)[conserved site], phospho-specific Rabbit Polyclonal

VP2701
Vav3 (a.a. 293-305) Rabbit Polyclonal

VX2485
Vav2 (a.a. 165-174) Blocking Peptide

New Releases
CK6760
image
Cadherin Family (E-, N-, and P-)
Antibody Sampler Kit
image
CK6740
image
VE-Cadherin Phospho-Regulation
Antibody Sampler Kit
image
FM0131
image
Fibronectin (Cell/Heparin2 Binding region)
Mouse Monoclonal
image